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I spent a good part of the day trying to figure out how long I can keep feces viable for testing microbes with. Contemplated how much information (in DNA) could be stored in an amazon snowmobile. https://t.co/jgIvAwE26w
If your umwelten doesn't include the information density of fecal waste, consider expanding your Lebenswelt today.https://t.co/DtTV5lFatF
I also found a $1700 fitbit clone that when combined with an EEG can theoretically predict your breathing.https://t.co/GqeRIdbDSmMood: https://t.co/cdhvITEatp
I found a solid state freezer design https://t.co/kzZNpmRHoSand a liquid radiator with no moving parts.https://t.co/JZa72uVLD3and have no idea what i'm doing.
I have no idea what I'm doing.https://t.co/r8MnXggTBr
My narrative structures are built up by a close personal relationship with my microbes.https://t.co/l6hvC59qhN
I was watching @YogscastLalna play @GreenHellGame and it got me wondering: how realistic their model of parasites and mushrooms? Does anybody know if there is a wiki for real-life analogues for stuff found in that game?Also, leaches edible IRL.https://t.co/CylDANHcVz
How I can get snap-freezing? Liquid Nitrogen?"In conclusion, the freezing process affected DNA yield and bacterial abundances, whereas snap freezing and storage temperature had only little influence on abundances of bacterial populations in pig feces."https://t.co/83f0gSNDvr
I wonder if replication depends on fecal quality. Time to find out bristol stool scales impact freezing rate of fecal matter. For reasons. https://t.co/x8ZVqKC0ue
Apparently there is a divergence in C diff infection detection via their toxins (enzyme immunoassays) vs detecting their DNA (nucleic acid amplification test ), the former varies depending on stool quality. DNA is persistent across stool quality scale tho.https://t.co/f8HEbOvnNQ
Tired: Building a freezer to store my fecal samples over the winter to show shifts in gut microbial populations reflect hydrogen sulfide levels, to prove I can hibernate.Wired: Building a freezer to show I can change the color of my poop, so I can shit rainbows like a unicorn.
Blue might be the hard one.https://t.co/6PurdjKwfA
Sometimes I joke. Sometimes I lie.Sometimes I wonder how much I'd need to pay @The_Lagrangian to build a deep learning based photo fecal analysis program.https://t.co/BlrIV3Uktd
@The_Lagrangian unironically samehttps://t.co/ZH3Ot1LmeK
@The_Lagrangian yethttps://t.co/98Z7q0BkSr
Dear twitter friends.With Ubiome having issues and generally only reporting down to genus. What would be the best way to run a large scale (6month+) bacterial assay that would tell me species level populations from poop, skin, and spit?https://t.co/Sr4pQCRjJy
I wonder how many bottles I'd need to store 183 days worth of piss samples. I should look up if I'll need to grade the pee based on sample timing. Do the first drips contain more metabolites?Been thinking about this a lot:https://t.co/mIWibvsoqR
Was thinking about ways to test it my self:. If I could pee on a plant to see how fast it sprouted; a way to approximate nitrogen content.Seems Ancient Egyptians thought you could test fertility by peeing on a pair of seedlings to see which grew faster.https://t.co/L2ECAaPnvK
I'm thinking about adding to my grant application about the risk of sloths coming and eating everything, depending on where I eventually end up doing the experiment.https://t.co/uxWCg0rBhw
Much more likely is the issue of storing 180 days of fecal waste being not exactly kosher for state laws. I may need to get an exception (or spend time to build a composting toilet and get a permit?)https://t.co/nJO77mO7km
Hello, Town of [redacted], Yes. Hi.I'm an autistic indepenant researcher trying to show humans can hibernate. Yes. I know. Well, I'm calling to ask about getting permission to store pee in large quantities. I promise I wont be using it to make gun powder. https://t.co/bbAjIVjsQh
I just realized that I have dangerous knowledge.https://t.co/vJ4a64vala
I found a food safe biodegradable bio-plastic disposable glove so I can handle my own fecal waste and do it Sustainably.Realized I can probably shit in freezer safe canning jars if I can't find a low-temp capable bio-plastic storage medium.Caveathttps://t.co/68wf86mwLR
TFW realizing I need to find out the expansion coefficient of diarrhea at extreme temperatures because of possibly flaring up crohn's symptoms.Just to balance this out, here's a video about mushrooms.https://t.co/xNH1Ts2Eu2 https://t.co/Cf2gKG1h2Q
Research notes include:https://t.co/FpwwmAmlBu
Ideas I have:Can I use the spectral intensity of lighting my farts on fire to get a rough idea of the kinds of gasses output from my feces?Can I ferment my pee to use it as a measure of my blood sugar?https://t.co/tPYYtLZQog
I want to diabetic testing kits that involves fermenting their pee and using a hydrometer + some machine learning to guess at their approximate blood sugar levels over sampling period.DIY insulin testing needs to exist alongside DIY insulin.Inspired:https://t.co/gbeDeLCwWX
I realized bulk storage & testing of effluvia is likely to be the largest hurdle in quest to run samples against my bathroom wastes; Am thinking about methodologies to deal with it as it is made.I learned 2 days in bed means I make about 2lbs of waste.https://t.co/MvkwC6hshB
If I lived on my own, it wouldn't take an hour between sampling. I'd have the scale right in the bathroom.Scale says it was a 1.9lbs difference. Most of it detected as fat. https://t.co/6SHyadY7t5
FYI total dissolved solids can be used as a proxy measure for kidney stone development and the impact of uric acid crystallization.https://t.co/oB7HwqptqZ
I don't think about it as risking my life, so much as de-risking it.Also, super useful if in testing hibernation "Unlike bears, once kidney damage occurs in humans, it is permanent with no options other than kidney transplants or long-term dialysis."https://t.co/0teVuUjXJF
"suggested that accumulated potassium loss by excitable tissue during torpor could potentially increase local extracellular potassium concentration, promote depolarization, and trigger an arousal thereby enabling a restoration of potassium balance"https://t.co/KydDv7wULl
I'm learning about acid corrosion to figure out if there are solenoids I can use to automate urine delivery for testing plant growth as a proxy measure for hormones.I have no idea what i'm doing with my life, but hydraulic manifolds sure are interesting.
"The rack is easy to setup and, with costs below $650, represents a useful alternative for research groups with limited financial resources."https://t.co/02WT3pPQ8R
Theoretically if I grow tomatoes hydroponically, I should be able to use automatic imaging techniques to detect nitrogen deficiency. I can perhaps differential concentrate it as a way to narrow down the amounts. https://t.co/JtvFh1WdFNWould stink real bad tho.
I just realized that if you have this condition and you pee in some grape juice it might turn into booze? New life goals.https://t.co/Y4KEJPOxIM
Ok... identifying the user via anal scan? Might be a bit too far.https://t.co/hUjtE9i5vAhttps://t.co/kmG2L7GzL5
In Pan Troglodytes, they use their face recognition area to recognize butts.I wonder if they outperform that toilet.https://t.co/J3vY2TOhx5
This, but its about toilets. https://t.co/P6h9ERFTHb
I'm living a cyberpunk novel. Buying products on a computer, having a system of networked couriers transport said items to my house. I open them up and proceed to hack and disable corporate surveillance subsystems, working on building my own when I can't. https://t.co/DvjpCAMPqq
Good news is that the dissolved solids seem to trend with water intake and match B2 vitamin concentration in a dose dependent manner. Did you know that Zero Water sensors can measure in the ~10k range? They have a little 'x10' thing on their display.https://t.co/hXY7gqOteP
Anybody know of a source of lab quality tools and accouterments on the same level as what McMaster-Carr is to engineering supply?I need to price out and plan space needed for durable glassware... for reasons.https://t.co/iqVXTNrBxY
If diabetic wiz taste like honey, why not make it into a mead?Turning it into whiskey is just silly."Once fermented [...] whisky blends are added to give colour, taste and viscosity, and the product is bottled with the name and age of the contributor."https://t.co/DijCr6Qgm2
Going from computational fluidics, to the nature of refactory glassware, and then musing how data transformations in flow-based and functional programming style are just alchemy."something like the glasswork rack [...], but for computation."https://t.co/icoUeACEEd https://t.co/rrgpYAjP0f
Thinking a lot about a study I read about people eating their own shit ("autologous FMT") as a way to mitigate antibiotic's impact on gut microbes. Merging in my head with ideas on behavior change.https://t.co/yKgn3Yu3DAIs this a 'brown pill'? asking for a friend.
“Digestion, Noun:— a person’s capacity to break down food into substances that can be used by the body”https://t.co/irPNkruHp8
If I wanted to blend a mixture of my own feces to get an accurate assay, do you think I should go with a @vejo? I suspect they'll be cancled b/c they're really another Juicero style VC bait piggy backing on the rise of the Keurig's subscription model.https://t.co/BlrIV3Uktd
Thinking about it, maybe I should just see if anyone has a portable blender that supports mason jars.https://t.co/fujKGoESQa
I just learned I can use process flow diagrams to model ecosystems.https://t.co/ByRnGDZsR2
And people wonder why I talk about artificial life so much.https://t.co/yK7rK3RGaw
Alan Turing and Von Neumann both died in the 1950s.I wonder if they wrote each other.https://t.co/aSsWXvnAoZ
"HT Odum's eMergy language" that BuildSoil talks about is perhaps so similar to electronic circuit diagrams in structure because at it's core they are both describing energy flows thru a system.This is also why I think fluidic computation is important.https://t.co/giydBxyndh
For future me: If you need glassware, consider looking at NileRed's videos on extracting capsacin. He has a home and a hobby chemist variations.https://t.co/lSsPoCmDF1
Digestion is just biochemistry happening in vivo.https://t.co/vg93CTif3K
For a comparison, Libgen and SciHub are about 100tb.https://t.co/xdiB0JTvdBAnd I think https://t.co/okENGgcGMt is in the 10s of Petabytes range. https://t.co/6kwbWOg5LQThis is a good first step! https://t.co/lHxAySkzba
When requesting funds for science experiments, modern sentiments necessitates at least a blurb about the environmental impact of your work.I can now safely say that all my waste can be recycled easily.https://t.co/FR5O8HDOVW
I'm basically larping as a ReplicAnt from @klei's #oxygennotincluded but IRL.https://t.co/jvPJJfKBYV
I produce about 0.7lbs of fecal waste and 1.2-1.8lbs of water depending on humidity and how much coffee I drank that day.It's surprisingly consistent unless I get sick. Then all bets are off.https://t.co/Mm6VQOyOmM
Oh wait, they're DuplicAnts. My bad.Anyway, this short animation is like many of my nightmares.https://t.co/vw2aB7F2Mw
If it weren't for the pandemic, I'd have so many jars of poop in a freezer right now.https://t.co/fwREMpR2eT
Another idea I am probably not going to be able to test properly due to the pandemic. But it's nice to dream.https://t.co/mNR6Z4pltM
I need a place I can send stool samples to to get clinical measurements of mucus and bowel related health.I think I can experimentally test this mucus emulsifier oddity with a formal double blind by creating 3 different recipes of Danish.https://t.co/PB42mruY97
"The strength of the associations between stool consistency and species richness, enterotypes and community composition emphasises the crucial importance of stool consistency assessment in gut metagenome-wide association studies."https://t.co/rTTMoRoLij
*puts notes about hardness and sheer strength on my list of tests to perform when I start freezing canning jars full of poop*TL;DR: is that a challenge?https://t.co/Q9REPIubqvhttps://t.co/OBuV6pMr7n
You can buy some fancy ones for $$$https://t.co/eUN77PYKhQ
Or you can just measure how far a ball bounces using a high framerate camera (after calibrating it with a kit), or a microphone! https://t.co/F2CrajXfdc
Measuring literal shit with homemade scientific instruments like one does.https://t.co/njTL2MKwLm
Oooh someone in the comments mentioned an actual testing design based on that "coefficient of restitution" (bounce) method.https://t.co/EM5XzrwfzC
So a piezo microphone sensor used as a meter to gauge the rebound hardness via scleroscope? easy."A scleroscope is a device used to measure rebound hardness. It consists of a steel ball dropped from a fixed height. The device was invented in 1907."https://t.co/ILHiwZAACO
I think I spend most of my time trying ot figure out how to make cheap instruments than actually doing anything useful with my life.https://t.co/xrz1G3hoDihttps://t.co/56S8g9xOgJ
This stuff is ripe for disruption.Here's a simple open source crowd based platform that, once you've got a device built to read spectra, you can just upload directly to as a public database: https://t.co/AC49Nr5tZRThese things aren't that hard to DIY https://t.co/Kyrx2qokGz
Well at least I'm in good company.https://t.co/YPSRUWRwsi https://t.co/04jZyoTD5y
"been searching basically ever since the World’s Fair for a way that I could use computers, math, and biology all together, and ideally physics and chemistry while you’re at it. Crystallography did that" —George McDonald Churchhttps://t.co/9dgAPV9VmhWhat could he mean by this?
I am probably going to die. soon.I don't care. If I am right, everyone I care about lives.https://t.co/9tHhZiheGv
I will keep my account locked.Too many want to imitate me. https://t.co/vuPx13kWGN
Do. NOT.https://t.co/d9GKNvkY2Z
Do not.https://t.co/p6C6sTHtsn
I'm retarded. Literally retarded.https://t.co/C3LNxHu1VE
Huge, if true:https://t.co/dce9424yXX
So if I want a reliable way to store large quantities of medical feces..."the Einstein-Szilard pump was later used for cooling breeder reactors, where its inherent reliability and safety were important."https://t.co/LRhtNuFnkO
Tired: smart toiletsWired: smart cities"can conceivably be undertaken at a population level in a treatment plant. That’s the basis of the field of study researchers are calling wastewater-based epidemiology." https://t.co/NeOcDmHdTihttps://t.co/BiniSZEQm2
@suchaone @__a__chimera__ There's a new tradition of divination using people's fecal wastes as input."The main benefits lie in the real-time aspect of the technology, providing insight into the diseases circulating in a community before the people affected know for themselves." https://t.co/OvuOLIWmqX
Divination Guidance."National Wastewater Surveillance System (NWSS) is currently ramping-up [...] information, including sampling, testing, and interpretation guidance, [...] will be updated on this page as they become available."https://t.co/sIVbKmRg9o
I found the forbidden crayon.https://t.co/HHXx9nZSJg https://t.co/X2F9gL2UeW
Crayon was a thing a doctors invented, but it didn't work well, likely because it was a cure-all that depended on self diagnosis. Didn't stop grifters from selling them.Excerpt from "The civiale remedial agency Urologic quackery in the gay nineties."https://t.co/7hprOfnv1v https://t.co/bE6BlQAd5q
The civiale remedial agency also sold a silk jockstrap that helps you if your ball sack's veins are bruised from an improperly fitted hernial truss.This is basically an early sears-roebucks catalog selling knock-off medical flim-flam.
oh cool, Gutenberg has the images!https://t.co/EYJrs1pHZFUnsurprisingly the drawing of the crayon is just a stick.But the ball compressor looks neat. https://t.co/852sJH7c8q
Seems a bit unnecessary to have an entire contraption like that when you can probably just use an actual jockstrap. https://t.co/6l2ruIHnqtBut... https://t.co/wawnzlzwmg
Its likely that due to fashions from the late Victorian era, you wouldn't have men walking around in supportive brief's like that.The trend was a union suit.https://t.co/T30I2JWmj0 https://t.co/JT7XVi837l
And while, according to this the jockstrap did exist, it was largely used for the new fangled Velocipede, (aka the 'boneshaker').https://t.co/IeVZDgUtK7
So this is a new goal for me now. I wanna make a forbidden golden crayon and see if it changes my poop color.https://t.co/JZUC5r0uSu
Seems like a good budding alchemist project. Involves bees wax bougies, some basic ant based chemistry to impart the colorhttps://t.co/i6igIElLjU
and a little bit more decoding of some Late Victorian Era influences on ideas around urology and sexual health.https://t.co/DwdMq2U0jC
He invented a machine that pumped yogurt into people's rectums. 😂> Dr. Kellogg believed that the bacteria used to make yogurt were protective against disease and “should be planted where they are most needed and may render the most effective service.” https://t.co/LZcTCSSMdV
"Capable of seeing individual atoms, commercial AFM prices range between US$10K and $1M, depending on the unit's features and capabilities [...] students succeeded in making a Lego-based AFM in five days at a cost less than $500." https://t.co/oACwm1bTgi https://t.co/uIm1UJHBaK
"Eventually, Temiz got fed up with the limitations of those boutique devices and built his own — and it only cost him $300 because he made it almost entirely out of Lego bricks.And it works"https://t.co/Spg6VDd2TM
"Soon after the glass micropipette was invented as a micro-tool for manipulation of single bacteria and the microinjection and microsurgery of living cells, it was seen to hold promise as a microelectrode to stimulate individual cells"https://t.co/fxIif0T25A
Hand forming micropipettes for electrodes? Gotta start somewhere. Might as well see what it would take to replicate @EmporiumThought's work with growing brain cells by recreating every discovery since the 1900s. https://t.co/BBLhva0ySI
Did you can just catch frogs and no one seems to care?https://t.co/anDlJhEFf5
There are complete instructions to make a micropippette by hand. Its not *that hard*. The challenging step is testing it.Dowben, R. M., & Rose, J. E. (1953). A Metal-Filled Microelectrode. Science, 118(3053), 22–24. doi:10.1126/science.118.3053.22
Trying to convince @nsreed that making glass electrodes by hand is a good idea.https://t.co/x4GjpUGUel
Ok, so..Biblically Accurate Tadpoles, but to study learning in autism.https://t.co/aAwh2CXZSthttps://t.co/haaaqwndEI
Be not afraid. 🐸https://t.co/M0xYmiBLci
Found a $23,000 machine that measures blood vessel reactivity that might let me find out how my smooth muscle tissue responds to changes in levels of chemicals in my blood.Gonna figure out how DIY, cuz it costs more than I've earned in the past 5 years. https://t.co/JXYQmd36iR
I love that I can spend like 3 minutes looking up papers and not even bother reading them and still can guess what they are doing to measure.https://t.co/aFtKS3oagw
Would be neat to find out if vascular reactivity corresponds to my ability to dead hang. https://t.co/ZbNT6v7VbG
Furiously looking up ways to store my own feces in vats so that I can avoid needing to eat dog feces ever again.https://t.co/Bd5GJp1lSx
Making a poop bank. Kinda like a spank bank, but for harvesting and storing seasonal variation in shit.https://t.co/nKC7qmQbFu
That shit was so cache'd."Experts at Harvard and elsewhere are proposing a new type of bank be set up nationwide: a poop bank." [2022]https://t.co/6F7oJsZBha
Gotta think bigger.https://t.co/2yU3FU4ucY
"Transplants prepared in maltodextrin-trehalose solutions, stored in a -80 °C standard freezer and then rapidly thawed at 37 °C, retained the best revivification potential" https://t.co/g96K2J4HE0
OpenBiome had some stats on the amount of shit they processed in a year."In 2018, the stool bank received 7,536 stool donations from 210 donors, a daily average of 20.6 donations, and processed 4,271 of those donations into FMT preparations." https://t.co/7dc5zkKrUq
Might be able to buy second hand shit storage stuff?"we made the difficult decision in November to begin phasing out manufacturing of new FMT treatments and sell equipment and related manufacturing assets." https://t.co/A2u9T45taj
Something is rotten in Denmark.https://t.co/74lpfq0VxN
> "The FDA does not claim that this is being done in the name of safety. Instead, an FDA official told Skonick that the goal was to "incentivize innovation without creating an access crisis."" https://t.co/LfcPaqRYFQhttps://t.co/uS8553876V https://t.co/ACKRx8sujo
On my todo list? A DIY cryocooler."I only reached -88C in this video, because a defective oil separator was causing oil to overflow into my heat exchanger and freeze at such low temperatures, resulting in clogs" https://t.co/cNT0vPr5Lphttps://t.co/0dJ8Sccp2E
The joke about a 'spank bank' earlier is that cryocoolers and the 'cold chain' process is also used to store and transport semen.it's also masturbation inuendo. https://t.co/JzVhjK9d6oA DIY cryofreezer may be useful for double doody and a double entendre.
Poetry on poop banks: https://t.co/UEkYlpsAlZ
McDonald's chicken nuggets made me excrete too much unprocessed information into the toilet, but it wasn't nearly as bad as Burger King's spicy variety.https://t.co/PGolaH7VCk
Biology was wrong; digestion is not a straight line from mouth to anus but a circle. Even in your gut, everything comes full circle. The ancient Greeks knew this. Hermeticism teaches this. When you eat, you are not just consuming calories, you are consuming information. And when you shit, you are not just excreting waste, you are excreting entropy. This is why it's so important to eat a variety of foods, to cultivate a diverse ecosystem in your gut. The health of your gut determines the clarity of your mind. This is why people who only eat chicken nuggets and Maccas are so fucking stupid. Anyway, I'm now going to take this concept to its logical conclusion: I'm going to eat my own shit. Wish me luck!